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Circulating Cell Free DNA Isolated and Amplified from the Plasma of Pancreatic Cancer Patients as Reference Material for ctDNA Assays
The validation and evaluation of circulating tumor DNA (ctDNA) assays poses analytical challenges because the amount of circulating cell-free DNA (ccfDNA) that can be obtained from a signle donor is very limited. A sustainable source of absolute standards is required to develop, optimize, and evaluate ctDNA assays.
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- How highly characterized, patient-like reference materials can help to establish concordance between laboratories in clinical trials
- Why amplifying ccfDNA enables production of the large quantities of high-quality reference materials needed for multiple analyses while maintaining original genotype profile
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