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Assessing Sensitivity of NGS RNA Fusion Assays Using a Multiplexed and Well-Characterized Linearity Panel

Many targeted NGS-based panels have been introduced to detect RNA fusions useful for prognosis and therapy selection in cancer.  Understanding the lower limits of detection (LOD) and the reproducibility across the reportable range of these assays is important for clinical adoption and regulatory compliance.

Watch the video and download this free poster to learn how we generated an RNA panel that represents decreasing levels of 16 different RNA fusions and exon-skipping events compared to the total cellular RNA. Fusion RNAs were sequence-verified and their concentrations measured by digital PCR (dPCR).

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